Botanicals in Ribes nigrum bud-preparations: an analytical fingerprinting to evaluate the bioactive contribution to...

Area of Science:

• Phytochemistry
• Analytical Chemistry
• Pharmacognosy

Background:

• Ribes nigrum L. (black currant) is a widely used herbal medicine known for its tonic and restorative properties.
• Current quality control for herbal extracts relies on measuring concentrations of main botanicals.
• Botanical fingerprinting is a recommended strategy for quality control of complex herbal mixtures by major Pharmacopeias.

Purpose of the Study:

• To conduct an analytical study of Ribes nigrum bud preparations.
• To identify and quantify major bioactive compounds in these preparations.
• To establish a chemical fingerprint for evaluating the contribution of different compound classes to the overall phytocomplex.

Main Methods:

• High-performance liquid chromatography with a diode-array detector (HPLC-DAD) was employed.
• Analyses were performed on both university laboratory and commercial Ribes nigrum bud preparations.
• Specific chromatographic methods were used for analyzing polyphenolic and terpenic compounds.

Main Results:

• Ribes nigrum bud preparations were confirmed as rich sources of anti-inflammatory and antioxidant compounds.
• The analytical fingerprint revealed significant levels of terpenic and polyphenolic compounds, notably catechins and phenolic acids.
• The study demonstrated the potential of these preparations as sources of health-promoting natural compounds.

Conclusions:

• Analytical fingerprinting is a valuable tool for assessing the chemical composition and bioactivities of plant-derived products.
• This research developed an effective method for botanical fingerprinting of Ribes nigrum bud preparations.
• The developed method serves as an important tool for quality control of herbal preparations.