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Related Concept Videos

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Related Experiment Video

Updated: May 9, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
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Published on: February 2, 2016

Development: Hippo signalling turns the embryo inside out.

Miguel Manzanares1, Tristan A Rodriguez

  • 1Cardiovascular Development and Repair Department, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain. mmanzanares@cnic.es

Current Biology : CB
|July 13, 2013
PubMed
Summary
This summary is machine-generated.

Cell position and signaling organization influence developmental lineage decisions. New research on the Hippo pathway in early mouse embryos reveals these mechanisms.

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Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Genetics

Background:

  • Lineage decisions in embryonic development are traditionally attributed to transcription factor activity.
  • Emerging evidence suggests that cell extrinsic factors, such as cell position and subcellular signaling organization, also play critical roles.

Purpose of the Study:

  • To investigate the role of cell position and subcellular signaling organization in lineage decisions during early mouse embryonic development.
  • To elucidate the mechanisms by which the Hippo pathway integrates positional cues for cell fate determination.

Main Methods:

  • Utilized live imaging techniques to track cell behavior and lineage in early mouse embryos.
  • Employed genetic manipulation to perturb Hippo pathway components and assess their impact on cell positioning and signaling.
  • Analyzed subcellular localization of key Hippo pathway proteins in relation to cell position.

Main Results:

  • Demonstrated that cell position within the early mouse embryo influences Hippo pathway activity.
  • Showed that subcellular organization of signaling components, particularly LATS kinases, is critical for regulating downstream targets like YAP/TAZ.
  • Identified specific cell-cell contact-dependent mechanisms that modulate Hippo pathway signaling based on position.

Conclusions:

  • Cell position and subcellular signaling organization are crucial, non-transcriptional regulators of lineage decisions in early mouse development.
  • The Hippo pathway acts as a key sensor of these physical cues, integrating them to control cell fate.
  • These findings expand our understanding of developmental mechanisms beyond transcription factor networks.