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Methylene blue protects against paraquat-induced acute lung injury in rats
Zhi-guang Tian1, Yong Ji, Wen-jing Yan
1Xuzhou Prevention and Treatment Hospital of Occupational Diseases, Xuzhou, Jiangsu, China.
Abstract:
Methylene blue (MB) attenuates acute lung injury (ALI) induced by liver or pancreas ischemia-reperfusion. But the protective effects of MB on paraquat (PQ)-induced ALI have not been elucidated. In this study, we aimed to study the effects of MB on ALI caused by PQ in rats. Male Sprague-Dawley rats were challenged acutely by PQ (25mg/kg, i.p.) with or without MB posttreatment (2mg/kg, i.p.) 2h after PQ administration. Lung specimens and the bronchoalveolar lavage fluid were isolated for pathological examinations and biochemical analyses 24h after PQ exposure. We found that the administration of MB significantly increased the activity of superoxide dismutase and decreased lung wet dry radio, the content of malondialdehyde and lactate dehydrogenase, the total cell number and the level of myeloperoxidase in bronchoalveolar lavage fluid. Pathological examination also revealed that MB alleviated PQ-induced histological damage. These results provide evidence that MB may be a new therapy in the treatment of PQ-induced ALI.
Insights
Methylene blue (MB) shows protective effects against paraquat (PQ)-induced acute lung injury (ALI) in rats. MB treatment reduced lung damage and inflammation markers, suggesting its potential as a therapeutic agent for PQ poisoning.
Area of Science:
- Toxicology
- Pulmonology
- Pharmacology
Background:
- Methylene blue (MB) is known to protect against acute lung injury (ALI) from ischemia-reperfusion.
- The protective effects of MB on paraquat (PQ)-induced ALI remain unclear.
Purpose of the Study:
- To investigate the therapeutic effects of Methylene blue (MB) on paraquat (PQ)-induced acute lung injury (ALI) in a rat model.
Main Methods:
- Male Sprague-Dawley rats received PQ (25mg/kg) followed by MB (2mg/kg) or vehicle 2h later.
- Lung tissues and bronchoalveolar lavage fluid were analyzed 24h post-exposure for pathological and biochemical markers.
Main Results:
- MB treatment significantly increased superoxide dismutase activity.
- MB decreased lung wet/dry ratio, malondialdehyde, lactate dehydrogenase, total cells, and myeloperoxidase in bronchoalveolar lavage fluid.
- Histological examination confirmed MB alleviated PQ-induced lung damage.
Conclusions:
- Methylene blue demonstrates significant protective effects against paraquat-induced acute lung injury in rats.
- MB may represent a novel therapeutic strategy for treating paraquat poisoning and associated ALI.