Related Experiment Video
Updated: May 9, 2026
08:22
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
A stable and sensitive voltammetric immunosensor based on a new non-enzymatic label
Rashida Akter1, Choong Kyun Rhee, Md Aminur Rahman
1Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
Biosensors & Bioelectronics
|July 13, 2013
Summary
A novel electrochemical immunosensor detects carcinoembryonic antigen (CEA) using a non-enzymatic label. This sensitive method offers a new tool for detecting CEA in serum samples.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biomedical Engineering
Background:
- Carcinoembryonic antigen (CEA) is a biomarker for various cancers.
- Development of sensitive and stable immunosensors is crucial for early cancer detection.
- Existing electrochemical methods often rely on complex enzymatic labels.
Purpose of the Study:
- To develop a stable and sensitive electrochemical immunosensor for CEA detection.
- To utilize a novel non-enzymatic label, 3, 3', 5, 5'-tetramethylbenzidine (TMB), for enhanced signal transduction.
- To employ a gold nanoparticles-deposited polydopamine (PD/AuNPs) platform for antibody immobilization and improved conductivity.
Main Methods:
- Fabrication of a PD/AuNPs platform for anti-CEA antibody immobilization.
- Conjugation of CEA with TMB to serve as an electrochemical label.
- Characterization of the immunosensor probe using scanning electron microscopy and electrochemical impedance spectroscopy.
- Detection of CEA via competitive binding using differential pulse voltammetry.
Main Results:
- The PD/AuNPs platform demonstrated enhanced conductivity, facilitating TMB redox reaction.
- The immunosensor exhibited high sensitivity for CEA detection, with a limit of detection of 10±0.6 pg/mL.
- The method showed selective detection of CEA and satisfactory recovery rates in spiked human serum samples.
Conclusions:
- A stable and sensitive electrochemical immunosensor for CEA detection was successfully developed.
- The use of TMB as a non-enzymatic label and the PD/AuNPs platform offers a promising approach for immunosensor design.
- The developed immunosensor shows potential for clinical application in CEA monitoring.
Keywords:
3, 3′, 5, 5′-TetramethylbenzidineCarcinoembryonic antigenElectrochemical immunosensorGold nanoparticlesNon-enzymatic labelPolydopamineMore Related Videos
Related Concept Videos
01:20Immunogold Electron Microscopy
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:20Voltammetry: Overview
Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...