Detection of the exercise-associated changes in serum proteins by two-dimensional electrophoresis under...

The changes in serum proteins caused by physical exercise (10-km run) were examined using two-dimensional electrophoresis under non-denaturing conditions. Two specific proteins were found to increase remarkably in amount. Both proteins had a slightly higher molecular mass than albumin, which suggests that they are albumin-bound with large amounts of sugars or lipids. However, these proteins were not adsorbed on an anti-albumin affinity column, and they were not stained by either periodic acid-Schiff base or Sudan Black. The molecular mass was determined to be 25,000 by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The changes of the specific proteins in competitors in a triathlon race were also examined.