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Teeth

Teeth

The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...

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Related Experiment Video

Updated: May 9, 2026

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

Published on: October 27, 2023

Incremental lines in mouse molar enamel.

Amer Sehic¹, Minou Nirvani, Steinar Risnes

¹Department of Oral Biology, Faculty of Dentistry, University of Oslo, P.O. Box 1052 Blindern, 0316 Oslo, Norway. amer.sehic@odont.uio.no

Archives of Oral Biology

|July 13, 2013

Summary

This summary is machine-generated.

Enamel incremental lines are visible in mouse molars, suggesting a daily rhythm in enamel formation. This finding supports the theory that circadian rhythms regulate tooth development.

Keywords:

Ameloblasts Dental enamel Molar Mouse Scanning electron microscopy

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Area of Science:

Developmental Biology
Dental Research
Histology

Background:

Enamel incremental lines provide insights into tooth development.
Understanding ameloblast activity rhythms is crucial for enamel formation.
Previous research has explored enamel structure and formation patterns.

Purpose of the Study:

Investigate the presence and periodicity of enamel incremental lines in mouse molars.
Examine the rhythm of ameloblast activity during mouse molar enamel formation.
Contribute to understanding the regulation of enamel development.

Main Methods:

Mouse molars were prepared using grinding and etching techniques.
Scanning electron microscopy was employed for detailed structural analysis.
Enamel incremental lines were identified and characterized based on their location and appearance.

Main Results:

Incremental lines appeared as distinct grooves, primarily in the cervical outer enamel (50-250μm from the junction).
Lines were most visible in the superficial prism-free layer and less so in deeper enamel with prism decussation.
In hypomineralized areas, lines were continuous from outer to inner enamel, with an average periodicity of ~4μm, decreasing towards the surface.

Conclusions:

Incremental lines are observable in mouse molar enamel, confirming layered apposition.
These lines likely represent a daily rhythm in enamel formation, regulated by the circadian clock.
The study supports the hypothesis that circadian rhythms play a role in regulating enamel development.