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Updated: May 9, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Polycyclic aromatic hydrocarbons stimulate human CYP3A4 promoter activity via PXR

Claudia Luckert1, Anke Ehlers, Thorsten Buhrke

  • 1Federal Institute for Risk Assessment, Department of Food Safety, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany.

Toxicology Letters
|July 13, 2013
PubMed

Keywords:
(±)-anti-11,12-dihydroxy-13,14-epoxy-11,12,13,14-tetrahydrodibenzo[a,l]pyrene(±)-anti-3,4-dihydroxy-1,2-epoxy-1,2,3,4-tetrahydrobenzo[c]phenanthrene(±)-anti-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene(±)-trans-11,12-dihydroxy-11,12-dihydrodibenzo[a,l]pyrene(±)-trans-3,4-dihydroxy-3,4-dihydrobenzo[c]phenanthrene(±)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene3-MC3-methylcholanthreneAhRBaPBaPDBaPDEBcPBcPDBcPDECARCYPCYP3A4 inductionDBDDBalPDBalPDDBalPDEDNA-binding domainDRERGSTLBDPAHPPARγPXRPXR responsive element(s)PXREPolycyclic aromatic hydrocarbonsReporter gene assayUASUDP-glucuronosyltransferase(s)UGTXREMaryl hydrocarbon receptorbenzo[a]pyrenebenzo[c]phenanthreneconstitutive androstane receptorcytochrome P(450) monooxygenase(s)dNRdibenzo[a,l]pyrenedirect repeatdistal nuclear receptor-binding element(s)everted repeatglutathione S-transferase(s)ligand-binding domainperoxisome proliferator-activated receptor γpolycyclic aromatic hydrocarbon(s)pregnane X receptorqRT-PCRreal-time quantitative PCRupstream activation sequencexenobiotic-responsive enhancer module

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