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Related Experiment Video

Updated: May 9, 2026

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

Closed-loop optogenetic intervention in mice.

Caren Armstrong1, Esther Krook-Magnuson1, Mikko Oijala1

  • 1139 Irvine Hall, Department of Anatomy & Neurobiology University of California, Irvine School of Medicine Irvine, CA 927697-1280 (949)824-3306 (phone) (949)824-9860 (fax).

Nature Protocols
|July 13, 2013
PubMed
Summary
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Researchers developed a real-time closed-loop system using optogenetic intervention to stop seizures in mice. This adaptable technology can respond to various brain states, offering new possibilities for neurological research and treatment.

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Optogenetics

Background:

  • Optogenetic interventions enable precise temporal control of neuronal activity in behaving animals.
  • A significant challenge for optogenetics is creating real-time responsive systems for unpredictable brain states and diseases.
  • Current methods lack the adaptability needed for dynamic neurological conditions.

Purpose of the Study:

  • To develop and validate a closed-loop system for real-time optogenetic intervention in neurological disorders.
  • To demonstrate the system's efficacy in stopping spontaneous seizures in a mouse model of epilepsy.
  • To provide a modular and adaptable platform for diverse brain state interventions.

Main Methods:

  • Construction of a closed-loop system integrating optogenetic control with real-time brain state detection.

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Related Experiment Videos

Last Updated: May 9, 2026

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
09:37

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

In Vivo Wireless Optogenetic Control of Skilled Motor Behavior
07:52

In Vivo Wireless Optogenetic Control of Skilled Motor Behavior

Published on: November 22, 2021

  • Development of custom downloadable software for closed-loop detection.
  • Testing the system's sensitivity and specificity in chronically epileptic mice.

    • Main Results:

    • The closed-loop optogenetic system successfully stopped spontaneous seizures with high sensitivity and specificity.
    • The system demonstrated real-time responsiveness to specific brain states.
    • The protocol is modular, allowing for adaptation to various interventions and brain states.

    Conclusions:

    • A novel closed-loop optogenetic system provides a powerful tool for real-time intervention in neurological conditions like epilepsy.
    • The developed system is highly sensitive, specific, and adaptable for diverse research applications.
    • This technology facilitates the study and potential treatment of unpredictable brain states.