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Updated: May 9, 2026

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and

Brian J Haas1, Alexie Papanicolaou2, Moran Yassour1,3

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA.

Nature Protocols
|July 13, 2013
PubMed
Summary

This study presents the Trinity platform for de novo transcriptome assembly from RNA-seq data, enabling genome-independent analysis. It offers tools for transcript abundance, differential expression, and protein-coding gene identification in non-model organisms.

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