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Updated: May 9, 2026
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Published on: May 11, 2017
Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and ploidy in
R Ranjan1, R K Singh, T Yasotha
1Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India.
Abstract:
This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.
