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Comparison of the 99th percentiles of three troponin I assays in a large reference population

Astrid Petersmann, Till Ittermann, Cornelia Fries

    Clinical Chemistry and Laboratory Medicine
    |July 13, 2013
    PubMed

    Insights

    Comparing sensitive troponin assays is crucial. This study found variations in 99th percentiles across assays, with some showing gender differences, highlighting the need for careful assay selection in clinical practice.

    Area of Science:

    • Clinical Chemistry
    • Cardiovascular Diagnostics
    • Biomarker Assays

    Background:

    • The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Task Force recommends comparing sensitive troponin assays.
    • A large, healthy reference population is essential for accurate assay evaluation.

    Purpose of the Study:

    • To compare three contemporary sensitive troponin I assays.
    • To determine the 99th percentiles for these assays in a large healthy population.
    • To investigate the influence of age and gender on troponin I levels.

    Main Methods:

    • Troponin I concentrations were measured using three sensitive assays in 2404 healthy individuals and a subgroup of 908.
    • Quantile regression was employed to calculate the 99th percentiles, accounting for the entire population distribution.
    • Statistical analysis explored age and gender dependencies for each assay.

    Main Results:

    • The 99th percentiles were 21 ng/L (ARCHITECT STAT), 31 ng/L (ADVIA Centaur), and 28 ng/L (Dimension Vista).
    • Gender differences in troponin I levels were observed for the Dimension Vista assay and the ARCHITECT assay subgroup.
    • No significant age dependency was found across the assays.

    Conclusions:

    • Quantile regression effectively estimates 99th percentiles and their relationship with demographic factors.
    • Assay-specific gender differences exist, necessitating consideration in clinical interpretation.
    • The findings support the IFCC recommendation for comparative assay evaluation.
    Abstract

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