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Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
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Host dependent iridoid glycoside sequestration patterns in Cionus hortulanus.

Christian Ulrich Baden1, Stephan Franke, Susanne Dobler

  • 1Molecular Evolutionary Biology, Department of Biology, Hamburg University, Hamburg, Germany. baden@evolbio.mpg.de

Journal of Chemical Ecology
|July 13, 2013
PubMed
Summary

Cionus hortulanus weevils

Area of Science:

  • Chemical ecology
  • Insect-plant interactions
  • Metabolic sequestration

Background:

  • Weevils in the genus Cionus sequester iridoid glycosides (IGs) from Scrophulariaceae plants.
  • Cionus hortulanus is unique in feeding on both Scrophularia and Verbascum genera.
  • Previous studies indicated host-dependent IG sequestration in C. hortulanus.

Purpose of the Study:

  • To determine if IG sequestration patterns in C. hortulanus are influenced by host plant availability or genetic population differences.
  • To investigate the metabolic plasticity of C. hortulanus in response to different host plants.

Main Methods:

  • Field collection of C. hortulanus from Scrophularia nodosa.
  • Laboratory rearing of collected weevils on either S. nodosa or Verbascum nigrum.

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  • Analysis of iridoid glycoside (aucubin and catalpol) concentrations in both host plants and weevils.
  • Main Results:

    • IG concentrations in C. hortulanus were host-plant specific, indicating metabolic adaptation rather than fixed genetic differences.
    • Weevils reared on S. nodosa showed higher aucubin than catalpol, mirroring their host plant.
    • Weevils reared on V. nigrum exhibited significantly higher concentrations of both aucubin and catalpol than their host, altering the catalpol to aucubin ratio.

    Conclusions:

    • The host plant significantly influences the iridoid glycoside profiles of Cionus hortulanus.
    • Data contradict genetically fixed sequestration differences, supporting host-driven metabolic regulation.
    • C. hortulanus demonstrates metabolic flexibility in sequestering and potentially modifying iridoid glycosides based on dietary intake.