[Rapid identification of 14 bile acids contained in Qingkailing injection by HPLC-ESI-MS/MS]
Jia-Yu Zhang1, Qian Zhang, Hong-Xia Zhang
1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangjiayu0615@163.com
Objective:
To investigate ESI-MS/MS fragmentation pattern of bile acids contained in Qingkailing injection, and the rapid identification method.
Method:
HPLC-ESI-MS/MS technology was adopted to investigate ESI-MS/MS fragmentation pattern of five bile acids contained in Qingkailing injection, and rapidly identify bile acids contained in Qingkailing injection from eight different manufacturers.
Result:
5 bile acids showed similar ESI-MS/MS fragmentation patterns, based on which 14 bile acids were rapidly separated from Qingkailing injection. Among them, 11 were found in injections of all of the manufacturers, and the rest three were found in individual manufacturers.
Conclusion:
HPLC-ESI-MS/MS is used to rapidly identify bile acids from complex material systems, which provides an effective method for interpreting the complex material base of compound traditional Chinese medicine preparations and enhance the quality standards.