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Updated: May 9, 2026

Cellular Redox Profiling Using High-content Microscopy
Published on: May 14, 2017
p66Shc, mitochondria, and the generation of reactive oxygen species
Mirella Trinei1, Enrica Migliaccio, Paolo Bernardi
1Department of experimental Oncology, European Institute of Oncology, Milan, Italy.
Abstract:
Reactive oxygen species (ROS), mainly originated from mitochondrial respiration, are critical inducers of oxidative damage and involved in tissue dysfunction. It is not clear, however, whether oxidative stress is the result of an active gene program or it is the by-product of physiological processes. Recent findings demonstrate that ROS are produced by mitochondria in a controlled way through specialized enzymes, including p66Shc, and take part in cellular process aimed to ensure adaptation and fitness. Therefore, genes generating specifically ROS are selected determinants of life span in response to different environmental conditions.
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