Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Introduction to Chemical Bonds01:01

Introduction to Chemical Bonds

Chemical Bonds
The electrons of the outermost energy level determine the energetic stability of the atom and its tendency to form chemical bonds with other atoms. The innermost electron shell has a maximum capacity of two electrons, but the next two electron shells can each have a maximum of eight electrons. This is known as the octet rule, which states that, with the exception of the innermost shell, atoms are most stable energetically when they have eight electrons in their valence shell, the...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Formation of Halohydrin from Alkenes02:41

Formation of Halohydrin from Alkenes

An alkene, such as propene, reacts with bromine in the presence of water to yield a halohydrin. Halohydrins contain a halogen and a hydroxyl group attached to adjacent carbons. When the halogen is bromine, it is called a bromohydrin, while a chlorohydrin has chlorine as the halogen.
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
Cohesion01:07

Cohesion

Cohesion is the attraction between molecules of the same type, such as water molecules. Water molecules have an overall neutral charge but are polar molecule. An oxygen atom in one water molecule has a partial negative charge that can bind to a hydrogen atom with a partial positive charge in a second water molecule, forming a hydrogen bond. Each water molecule can form up to four hydrogen bonds with other water molecules. Hydrogen bonds are responsible for water's cohesive nature.
On a surface,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zebrafish macrophages convert physical wound signals into rapid vascular permeabilization.

Nature communications·2026
Same author

Mechanotransduction by nuclear envelope tension.

Nucleus (Austin, Tex.)·2025
Same author

Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction.

Nature cell biology·2025
Same author

Fibroblasts promote osmotic surveillance by wound-induced unique calcium patterns.

The Journal of cell biology·2025
Same author

The G-protein coupled receptor OXER1 is a tissue redox sensor essential for intestinal epithelial barrier integrity.

bioRxiv : the preprint server for biology·2025
Same author

DHRS7 Integrates NADP<sup>+</sup>/NADPH Redox Sensing with Inflammatory Lipid Signalling via the Oxoeicosanoid Pathway.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 9, 2026

High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye
05:16

High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye

Published on: January 19, 2024

H2O2: a chemoattractant?

Balázs Enyedi1, Philipp Niethammer

  • 1Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.

Methods in Enzymology
|July 16, 2013
PubMed
Summary
This summary is machine-generated.

Hydrogen peroxide (H2O2) is investigated for its role in innate immunity. New methods in zebrafish allow studying how H2O2 influences leukocyte chemotaxis during wound healing.

Keywords:
ChemotaxisDUOXH(2)O(2)HyPerLeukocyteWoundZebrafish

More Related Videos

Two Types of Assays for Detecting Frog Sperm Chemoattraction
10:02

Two Types of Assays for Detecting Frog Sperm Chemoattraction

Published on: December 27, 2011

Related Experiment Videos

Last Updated: May 9, 2026

High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye
05:16

High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye

Published on: January 19, 2024

Two Types of Assays for Detecting Frog Sperm Chemoattraction
10:02

Two Types of Assays for Detecting Frog Sperm Chemoattraction

Published on: December 27, 2011

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Reactive oxygen species, such as hydrogen peroxide (H2O2), are increasingly recognized for their roles in cellular signaling.
  • H2O2 has been implicated in leukocyte recruitment to wounds and tumors in zebrafish, but its precise mechanism remains unclear.
  • Understanding H2O2's function is crucial for elucidating innate immune responses.

Purpose of the Study:

  • To establish experimental protocols for investigating the role of H2O2 in leukocyte chemotaxis.
  • To provide methods for studying H2O2's direct chemoattractant properties or its influence on existing chemoattractant pathways.
  • To enable in vivo analysis of H2O2's involvement in innate immune cell migration in a vertebrate model.

Main Methods:

  • Detailed protocol for the zebrafish tail fin wounding assay to analyze leukocyte chemotaxis in vivo.
  • Method for measuring H2O2 levels in live zebrafish larvae using the genetically encoded HyPer sensor.
  • Utilization of wide-field and spinning disk confocal microscopy for H2O2 measurement.

Main Results:

  • The study outlines foundational methods for H2O2 research in vivo.
  • The described protocols facilitate the analysis of leukocyte migration in response to H2O2.
  • The methods allow for the quantification of H2O2 dynamics during immune responses.

Conclusions:

  • The developed experimental procedures provide a basis for dissecting the role of H2O2 in leukocyte chemotaxis.
  • These methods can be applied to understand H2O2-mediated innate immune responses in a vertebrate system.
  • Further research using these protocols will clarify H2O2's contribution to immune cell recruitment and wound healing.