Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Protein Maturation

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Human Disposition, Metabolism, and Excretion of Sevasemten (EDG-5506), a Selective Modulator of Fast Myosin in Healthy Volunteers.

Journal of clinical pharmacology·2026

Same author

Effects of sevasemten (EDG-5506) on safety, biomarkers, and functional measures in adults with Becker muscular dystrophy: results of a phase 1b, open-label study.

EBioMedicine·2026

Same author

Oxygen-Tolerant Photo-Induced Miniaturized Accelerated Atom Transfer Radical Polymerization (OPTIMA-ATRP) for High-Throughput Synthesis of Polymer Bioconjugates.

Angewandte Chemie (International ed. in English)·2026

Same author

Controlled Synthesis of Liquid-Crystalline Polymers Under Ambient Conditions by Red-Light-Driven ATRP.

Macromolecules·2026

Same author

Dual-Pathway Strategy for Click-Type Functionalization and Programmable Polymer Deconstruction.

Macromolecules·2026

Same author

Open-Vessel and Scalable Synthesis of Linear and Branched Poly(meth)acrylic Acid via Light-Mediated Atom Transfer Radical Polymerization in Water.

Macromolecules·2026

Same journal

Corrigendum to "Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide" [Biomaterials 42 (2015) 16442].

Biomaterials·2026

Same journal

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026

Same journal

Peptide coacervate-mediated siRNA delivery for dual PD-1/PD-L1 blockade to enhance colorectal cancer immunotherapy.

Biomaterials·2026

Same journal

A sonosensitizing hydrogel with tumour-confined stability for intrinsically targeted sonodynamic therapy.

Biomaterials·2026

Same journal

Multidimensional intestinal barrier repair strategies for alleviating inflammatory bowel disease and gut-liver axis-associated metabolic liver disease.

Biomaterials·2026

Same journal

A dual-twisted molecular strategy achieves dramatic quantum-yield enhancement in NIR-II AIEgen for high-performance bioimaging.

Biomaterials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 9, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Tailoring enzyme activity and stability using polymer-based protein engineering.

Chad Cummings¹, Hironobu Murata, Richard Koepsel

¹Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States.

|July 16, 2013

Summary

This summary is machine-generated.

Polymer-based protein engineering enhances enzyme stability and function using temperature-responsive polymers. This method modifies enzyme properties without genetic manipulation, offering predictable control over enzyme kinetics.

Keywords:

Atom transfer radical polymerization Chymotrypsin Enzyme Enzyme-polymer conjugates Protein engineering

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Related Experiment Videos

Last Updated: May 9, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Area of Science:

Biotechnology
Polymer Chemistry
Enzyme Engineering

Background:

Polymer-based protein engineering (PBPE) provides a non-mutagenic route to modify enzyme characteristics.
Stimuli-responsive polymers can alter enzyme activity and stability based on environmental cues.
Existing methods often rely on complex molecular biology techniques.

Purpose of the Study:

To demonstrate the effectiveness of temperature-responsive polymers in enhancing enzyme stability and function.
To explore the synthesis of enzyme-polymer conjugates using a "grafting from" approach.
To show predictable control over enzyme kinetics and stability via PBPE.

Main Methods:

Synthesized enzyme-polymer conjugates using atom transfer radical polymerization (ATRP).
Attached a water-soluble initiator to chymotrypsin's surface amines, creating a macroinitiator.
Grew poly(N-isopropylacrylamide) and poly[N,N"-dimethyl(methacryloylethyl) ammonium propane sulfonate] from the modified chymotrypsin.

Main Results:

Enzyme-polymer conjugates exhibited increased stability across a broad temperature range.
Conjugates retained temperature-dependent conformational changes and enzyme function.
Bioactivity and substrate affinity were maintained post-conjugation.

Conclusions:

PBPE offers a predictable method to enhance enzyme stability and function.
Temperature-responsive polymers can be effectively grafted onto enzymes to improve performance.
This approach bypasses the need for molecular biology-based mutagenesis for enzyme modification.