Experimental aerosol Mycobacterium bovis model of infection in goats

The use of animal models is essential in testing the efficacy for novel therapies against tuberculosis (TB). Calves and non-human primates are examples of large animal models currently used to test TB vaccine efficacy but these animals are difficult and very expensive to house under high containment conditions. The goat may represent an effective but less expensive alternative for testing prototype vaccines against TB. Goats are susceptible to Mycobacterium bovis, Mycobacterium caprae and Mycobacterium tuberculosis infection. Aerosolized bacteria are the most common source of natural infection in humans and the primary site of natural infection is the respiratory tract. We developed a simple procedure for infecting goats with M. bovis by aerosol exposure. After 8 and 12 weeks of infection the goats were euthanized, post-mortem analysis was performed, and all exposed animals presented TB compatible lesions in the lung and associated lymph nodes. Selected lung lesions and respiratory lymph nodes were evaluated and cultured for bacteriological and histological analysis. The present work shows a reliable new animal model of aerosol infection to be used in the understanding of TB disease and development of new therapies.