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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: May 9, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

Development and validation of three Capripoxvirus real-time PCRs for parallel testing.

A Haegeman¹, K Zro, F Vandenbussche

¹CODA-CERVA, Viral Diseases, Vesicular and Exotic Diseases, Groeselenberg 99, B-1180 Brussels, Belgium.

Journal of Virological Methods

|July 16, 2013

Summary

This study developed a highly sensitive and specific triplet real-time PCR panel for accurate Capripoxvirus detection. This diagnostic tool aids in controlling emerging Capripoxvirus outbreaks and their socio-economic impact.

Keywords:

Capripoxvirus Diagnostics Parallel testing Real-time PCR Validation

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Last Updated: May 9, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

Area of Science:

Veterinary Virology
Molecular Diagnostics
Emerging Infectious Diseases

Background:

Capripoxviruses cause significant socio-economic losses and are emerging threats.
Effective disease control relies on accurate diagnostic tools for pathogen identification.
Real-time PCR offers high sensitivity and specificity for pathogen detection, including Capripoxviruses.

Purpose of the Study:

To develop a triplet real-time PCR panel for Capripoxvirus detection.
To ensure the panel exhibits high sensitivity and specificity.
To provide validation data for parallel use of the diagnostic panel.

Main Methods:

Development of a multiplex real-time PCR assay.
Evaluation of analytical and diagnostic performance characteristics.
Validation of a triplet real-time PCR panel for Capripoxvirus identification.

Main Results:

A highly sensitive and specific triplet real-time PCR panel was successfully developed.
The panel demonstrated robust performance characteristics suitable for diagnostic applications.
The study provides data supporting the parallel implementation of this diagnostic tool.

Conclusions:

The developed triplet real-time PCR panel is a valuable tool for Capripoxvirus diagnosis.
This panel can enhance the accuracy and efficiency of Capripoxvirus detection and surveillance.
Implementation of this panel supports disease control and eradication efforts.