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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

New type of antimicrobial protein produced by the plant pathogen Clavibacter michiganensis subsp. michiganensis

Zhanliang Liu1, Ping Ma, Ingrid Holtsmark

  • 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

Applied and Environmental Microbiology
|July 16, 2013
PubMed

Insights

A novel protein, Clavibacter michiganensis subsp. michiganensis AMP-I (CmmAMP-I), inhibits potato ring rot bacteria. This newly identified bacteriocin shows high activity and may combat the disease effectively.

Area of Science:

  • Plant Pathology
  • Bacteriology
  • Biochemistry

Background:

  • Clavibacter michiganensis subsp. michiganensis (Cmm) produces a protein inhibiting Clavibacter michiganensis subsp. sepedonicus (Cms).
  • Cms causes bacterial ring rot, a significant potato disease.

Purpose of the Study:

  • Identify the gene encoding the Cmm-secreted inhibitory protein, CmmAMP-I.
  • Recombinantly produce and characterize CmmAMP-I for its antimicrobial properties and potential applications.

Main Methods:

  • Gene identification using tryptic fragment sequences.
  • Recombinant protein production with an N-terminal intein tag.
  • Antimicrobial activity assays to determine IC50 and toxicity.

Main Results:

  • The gene encoding CmmAMP-I was identified, revealing a Sec-dependent signal peptide.
  • Recombinant CmmAMP-I exhibited high antimicrobial activity (IC50 ~10 pmol).
  • The protein showed no toxicity to potato tissues and represents a novel bacteriocin class.

Conclusions:

  • CmmAMP-I is a new type of bacteriocin with potent activity against Cms.
  • Its narrow spectrum and lack of phytotoxicity make it a promising candidate for controlling potato bacterial ring rot.

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