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Updated: May 9, 2026

Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Engineering nucleases for gene targeting: safety and regulatory considerations
Katia Pauwels1, Nancy Podevin, Didier Breyer
1Scientific Institute of Public Health, Biosafety and Biotechnology Unit, J. Wytsmanstraat 14, Brussels B-1050, Belgium.
Abstract:
Nuclease-based gene targeting (NBGT) represents a significant breakthrough in targeted genome editing since it is applicable from single-celled protozoa to human, including several species of economic importance. Along with the fast progress in NBGT and the increasing availability of customized nucleases, more data are available about off-target effects associated with the use of this approach. We discuss how NBGT may offer a new perspective for genetic modification, we address some aspects crucial for a safety improvement of the corresponding techniques and we also briefly relate the use of NBGT applications and products to the regulatory oversight.
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