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Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention
Yoshiaki Takei1, Atsushi Murata, Kento Yamagishi
1Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, 02C227, TWIns, Tokyo, 162-8480, Japan.
Chemical Communications (Cambridge, England)
|July 16, 2013
Abstract:
The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca(2+) indicator in the cytosol. Specifically, a novel clickable Ca(2+) indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca(2+) oscillation was observed for an extended period of time.
