Related Experiment Video
Updated: May 9, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
Proline iminopeptidase PepI overexpressing Lactobacillus casei as an adjunct starter in Edam cheese
Sahar Navidghasemizad1, Timo M Takala1, Tapani Alatossava1
1Department of Food and Environmental Sciences; University of Helsinki; Helsinki, Finland.
Abstract:
In this study the growth of genetically modified Lactobacillus casei LAB6, overexpressing proline iminopeptidase PepI and its capacity to increase free proline was investigated during ripening of Edam cheese. The strain successfully survived 12 weeks of ripening period in cheese. The food-grade plasmid pLEB604, carrying the pepI gene, was stable, and PepI enzyme was active in LAB6 cells isolated at different stages of the ripening process. However, HPLC analyses indicated that Lb. casei LAB6 could not increase the amount of free proline in ripened cheese.
Related Concept Videos
Microbes in Food Production
Microbes in the Production of Fermented Foods
Production of Organic Acids
Production of Pharmaceuticals
Production of Antibiotics
Probiotics

