Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamines--potential nucleating factors in bile.

F Konikoff1, G Goldman, Z Halpern

  • 1Department of Gastroenterology, Ichilov Hospital, Tel Aviv Medical Center, Israel.

Liver
|June 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A retrospective cohort study comparing pregnancy outcomes and neonatal characteristics between HIV-infected and HIV-non-infected mothers.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2020
Same author

Corrigendum: methotrexate in chronic active Crohn's disease: a double-blind, randomized, israeli multicenter trial.

The American journal of gastroenterology·2015
Same author

Oral administration of non-absorbable delayed release 6-mercaptopurine is locally active in the gut, exerts a systemic immune effect and alleviates Crohn's disease with low rate of side effects: results of double blind Phase II clinical trial.

Clinical and experimental immunology·2015
Same author

Characterization of novel CD55 isoforms expression in normal and neoplastic tissues.

Tissue antigens·2013
Same author

Drug Trials in Inflammatory Bowel Diseases 1993-1995: A Survey Conducted by the IOIBD.

Inflammatory bowel diseases·2013
Same author

Prospective multicenter performance evaluation of the second-generation colon capsule compared with colonoscopy.

Endoscopy·2009
Same journal

Ubiquitin is a possible new predictive marker for the recurrence of human hepatocellular carcinoma.

Liver·2002
Same journal

Hepatic graft-versus-host disease resembling acute hepatitis: additional treatment with ursodeoxycholic acid.

Liver·2002
Same journal

Allergic cholestatic hepatitis and exanthema induced by metamizole: verification by lymphocyte transformation test.

Liver·2002
Same journal

Macro-aspartate aminotransferase in a female with antibodies to hepatitis C virus.

Liver·2002
Same journal

Cytotoxic response of sinusoidal endothelial cells to polymorphonuclear leukocytes and its potential implication in hypoxia-reoxygenation injury.

Liver·2002
Same journal

Early gene expression of hepatocyte growth factor in mononuclear phagocytes of rat liver after administration of carbon tetrachloride.

Liver·2002
See all related articles

Polyamines like spermine and spermidine act as nucleating agents in human bile, influencing cholesterol crystal formation. This study highlights the role of non-protein compounds in bile lithogenicity.

Area of Science:

  • Biochemistry
  • Gastroenterology
  • Bile Acid Research

Background:

  • Bile lithogenicity is determined by cholesterol saturation and nucleating/antinucleating factors.
  • Research has primarily focused on biliary proteins, especially glycoproteins, as key factors.
  • The role of non-protein compounds in bile nucleation remains less understood.

Purpose of the Study:

  • To investigate the potential nucleating effect of specific polyamines (spermine, spermidine, cadaverine, putrescine) in human bile.
  • To assess the dose-dependent nature of this effect.
  • To explore the implications of these findings for understanding cholesterol nucleation in bile.

Main Methods:

  • Performed 183 mixing experiments using both model bile and native human bile from 10 patients.

Related Experiment Videos

  • Assessed the nucleating effect of polyamines at varying concentrations.
  • Analyzed cholesterol distribution between vesicular and micellar carriers.

    • Main Results:

    • Spermine, spermidine, cadaverine, and putrescine demonstrated a significant nucleating effect in both model and native human bile.
    • The observed nucleation effect was dose-dependent, particularly at concentrations up to 10 mmol/l.
    • No shift in cholesterol distribution between carriers was associated with the polyamine-induced nucleation.

    Conclusions:

    • Polyamines (spermine, spermidine, cadaverine, putrescine) act as nucleating agents in human bile.
    • These non-protein compounds play a potentially important role in the cholesterol nucleation process.
    • Further research is needed to determine if these effects are pharmacologic or physiological.