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DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions

Tetsuro Komatsu1, Takeshi Sekiya, Kyosuke Nagata

  • 1Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8575, Japan.

Scientific Reports
|July 16, 2013
PubMed

Insights

CTCF is essential for adenovirus DNA replication and late gene expression during infection. This protein binds to viral chromatin, regulating crucial functions in the later stages of the viral life cycle.

Area of Science:

  • Virology
  • Molecular Biology
  • Epigenetics

Background:

  • Adenovirus late gene expression is dependent on viral DNA replication.
  • The precise mechanism regulating this process remains unclear.
  • Understanding these regulatory mechanisms is crucial for controlling viral infections.

Purpose of the Study:

  • To investigate the role of CTCF (CCCTC-binding factor) in adenovirus replication and gene expression.
  • To elucidate the mechanism by which CTCF influences viral genome functions.

Main Methods:

  • Utilized knockdown techniques to reduce CTCF levels in infected cells.
  • Assessed the impact of CTCF knockdown on viral DNA replication and gene expression (early vs. late).
  • Performed chromatin immunoprecipitation (ChIP) assays to determine CTCF binding to viral chromatin.

Main Results:

  • Knockdown of CTCF significantly suppressed adenovirus DNA replication.
  • CTCF depletion specifically inhibited late gene expression, while early gene expression remained unaffected.
  • ChIP assays revealed that CTCF binds to viral chromatin in a manner dependent on viral DNA replication.

Conclusions:

  • CTCF is a critical regulator of adenovirus genome functions during the late stages of infection.
  • CTCF's role is linked to its ability to bind viral chromatin, which is dependent on viral DNA replication.
  • These findings provide new insights into the molecular mechanisms governing adenovirus gene expression and replication.

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