Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SERS on analyte-enriched blood for rapid, culture-free sepsis recognition and causative pathogen identification with super operational neural networks.

Talanta·2026
Same author

Dynamic and precise electromagnetic levitation of single cells.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Dynamics of Pulsed-Laser Interaction with Janus Particles.

ACS photonics·2025
Same author

Measuring the effect of repetitive stretching on the deformability of human red blood cells using optical tweezers.

Scientific reports·2025
Same author

Accuracy verification of protoacoustic measurements in a heterogeneous phantom by an optical hydrophone.

Medical physics·2024
Same author

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging.

Biomedical optics express·2024
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: May 9, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Virtual source method for diffuse optical imaging.

Hakan Erkol1, Mehmet Burcin Unlu

  • 1Department of Physics, Bogazici University, Bebek, Istanbul, Turkey. hakan.erkol@boun.edu.tr

Applied Optics
|July 16, 2013
PubMed
Summary
This summary is machine-generated.

Researchers retrieved the diffusive Green's function using virtual light sources, potentially replacing noisy measurements in optical tomography. This method can improve accuracy in small animal and human studies and reduce imaging time.

More Related Videos

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Related Experiment Videos

Last Updated: May 9, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Area of Science:

  • Biomedical optics
  • Wave propagation
  • Computational physics

Background:

  • Diffusive wave propagation is crucial in fields like optical tomography.
  • Accurate Green's functions are essential for modeling light transport.
  • Current methods can be limited by noisy measurements and long imaging times.

Purpose of the Study:

  • To retrieve the diffusive Green's function using virtual light sources.
  • To demonstrate the feasibility of this method in optical tomography.
  • To explore applications in reducing noise and imaging time.

Main Methods:

  • Utilized Robin boundary conditions and representation theorems for diffusive media.
  • Calculated the diffusive Green's function by combining detector readings and virtual light sources.
  • Performed 2D simulations using the finite element method.

Main Results:

  • Demonstrated the feasibility of using virtual light sources in a simulated circular region.
  • Showcased the potential to replace noisy measurements with synthetic data.
  • Indicated a possibility for reduced imaging times.

Conclusions:

  • The virtual source method offers a promising approach for enhancing optical tomography.
  • This technique has significant potential for applications in small animal and human studies.
  • Further development could lead to more accurate and efficient imaging.