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Updated: May 9, 2026

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Correlative microscopy for 3D structural analysis of dynamic interactions.

Sangmi Jun¹, Gongpu Zhao, Jiying Ning

¹Department of Structural Biology, University of Pittsburgh School of Medicine, USA.

Journal of Visualized Experiments : Jove

|July 16, 2013

Summary

This summary is machine-generated.

This study introduces a correlative imaging method combining live-cell microscopy and cryo-electron tomography (cryoET) to visualize early HIV-1 infection events. This approach enables high-resolution structural analysis of virus-host cell interactions in their native environment.

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Area of Science:

Structural Biology
Virology
Cell Biology

Background:

Cryo-electron tomography (cryoET) offers molecular resolution of cellular structures in near-physiological states.
Visualizing dynamic viral entry, like HIV-1, within host cells using cryoET is challenging due to infrequent events.
Live-cell imaging provides dynamic data but lacks the high resolution of cryoET.

Purpose of the Study:

To develop a correlative method for visualizing early HIV-1 infection events at molecular resolution.
To combine live-cell imaging with cryo-fluorescent microscopy and cryoET for accurate structural analysis.
To enable detailed investigation of virus-host cell interactions and other dynamic cellular processes.

Main Methods:

Developed a correlative imaging approach integrating live-cell fluorescent microscopy, cryo-fluorescent microscopy, and cryoET.
Utilized live-cell and cryo-fluorescent signals to guide cryoET sampling with high precision.
Applied confocal live-cell microscopy to identify HIV-1 infected HeLa cells, followed by cryoET for 3D structural analysis.

Main Results:

Successfully visualized early HIV-1 infection events by correlating live-cell imaging with high-resolution cryoET.
Demonstrated the ability to obtain structural information complemented by dynamic functional data from fluorescently labeled targets.
Established a robust protocol for structural investigation of HIV-1 and host-cell interactions.

Conclusions:

The developed correlative imaging technique allows for unprecedented visualization of virus-host cell dynamics.
This method enhances structural insights by combining high-resolution cryoET with live-cell imaging data.
The approach has broad applicability for studying dynamic cellular processes beyond viral infections, including cell signaling and membrane trafficking.