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Nuclear factor one X regulates Bobby sox during development of the mouse forebrain
Chantelle Dixon1, Tracey J Harvey, Aaron G Smith
1The School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
Cellular and Molecular Neurobiology
|July 16, 2013
Summary
Nuclear Factor One X (NFIX) controls forebrain development. This study identifies Bobby Sox (Bbx) as a downstream target, revealing NFIX represses Bbx expression in developing neocortex and hippocampus progenitor cells.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- Nuclear Factor One X (NFIX) is crucial for neocortex and hippocampus development.
- NFIX regulates astrocyte-specific gene expression and progenitor pathways.
- Transcriptional targets of NFIX in cortical development are not fully understood.
Purpose of the Study:
- To identify downstream transcriptional targets of NFIX during forebrain development.
- To investigate the role of Bobby Sox (Bbx) as a target of NFIX.
Main Methods:
- Analysis of gene expression in Nfix (-/-) mice.
- Reporter assays to assess NFIX's effect on Bbx promoter activity.
Main Results:
- Bobby Sox (Bbx) is expressed in developing neocortex and hippocampus progenitor cells.
- Bbx expression is upregulated in Nfix (-/-) mice.
- NFIX directly represses Bbx promoter-driven expression.
Conclusions:
- Bbx is a downstream transcriptional target of NFIX.
- NFIX negatively regulates Bbx expression during forebrain development.
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