Experimental gastric cancer induced by N-methyl-N'-nitro-N-nitrosoguanidine in rats

MNNG-induced experimental gastric cancer developed in the pyloric glandular region. Many of the tumors produced were well or moderately differentiated adenocarcinomas of elevated type. According to the degree of depth invasion, a majority of the tumors were classified as early-stage lesions invading within submucosal layer. There were two poorly differentiated lesions, which were both recognized as serosal neoplastic involvements. The tumors were positively stained by toluidine blue (pH 4.1) to indicate the presence of stroma. The Brdu-LI determined for gastric mucosal epithelial cells was significantly higher in the MNNG-treated group than in the untreated control group. However, the LI for cancer tissue in the MNNG-treated group did not significantly differ from that for gastric mucosa in the same group.