Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ABCA1-Mediated Structural Diversity of HDL Subspecies and Their Proposed Roles in Cardioprotection.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Conformational Dynamics of hAgo2 Silencing: Decoding Functional Divergence across Human Argonaute Paralogs.

Journal of chemical information and modeling·2025
Same author

Isotopic evidence reveals the gradual intensification of millet agriculture in Neolithic western Loess Plateau.

Fundamental research·2025
Same author

Lecithin:cholesterol acyltransferase binds a discontinuous binding site on adjacent apolipoprotein A-I belts in HDL.

Journal of lipid research·2025
Same author

Predicting and interpreting EPR spectra of POPC lipid bilayers with transmembrane α-helical peptides from all-atom molecular dynamics simulations.

Physical chemistry chemical physics : PCCP·2025
Same author

APOA2 increases cholesterol efflux capacity to plasma HDL by displacing the C-terminus of resident APOA1.

Journal of lipid research·2024

Related Experiment Video

Updated: May 9, 2026

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
11:13

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

MD simulations suggest important surface differences between reconstituted and circulating spherical HDL.

Jere P Segrest1, Martin K Jones, Andrea Catte

  • 1Department of Medicine and Center for Computational and Structural Dynamics, University of Alabama at Birmingham, Birmingham, AL 35294.

Journal of Lipid Research
|July 17, 2013
PubMed
Summary

Molecular dynamics simulations reveal that reconstituted HDL particles contain excess phospholipids, unlike natural HDL. This finding highlights differences between in vitro models and circulating HDL structures.

Keywords:
apolipoprotein A-Ihigh density lipoproteins subspeciesmolecular dynamics simulationspheroidal HDL

More Related Videos

Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Related Experiment Videos

Last Updated: May 9, 2026

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
11:13

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • High-density lipoprotein (HDL) particles are crucial for reverse cholesterol transport.
  • Spheroidal HDL (sHDL) particles are dynamic and their structures are challenging to model.
  • Molecular dynamics (MD) simulations offer a powerful tool for investigating HDL structure and dynamics.

Purpose of the Study:

  • To compare the structural characteristics of reconstituted sHDL with circulating human HDL using MD simulations.
  • To investigate the influence of lipid and apolipoprotein composition on sHDL structure.
  • To assess the fidelity of reconstituted sHDL as models for circulating HDL.

Main Methods:

  • Utilized molecular dynamics (MD) simulations to model sHDL particles.
  • Simulated reconstituted sHDL and circulating human HDL with varying apolipoprotein A-I (apoA-I) stoichiometries.
  • Analyzed lipid and protein content, focusing on core and surface lipid distribution.

Main Results:

  • MD simulations showed rapid remodeling of discoidal HDL to sHDL with a cholesteryl ester/triglyceride (CE/TG) core.
  • Circulating sHDL contained similar core lipid volumes but significantly less surface lipid compared to reconstituted sHDL with the same apoA-I number.
  • Reconstituted sHDL were found to contain kinetically trapped excess phospholipids.

Conclusions:

  • In vitro reconstituted HDL are imperfect models for circulating sHDL due to excess phospholipids.
  • Phospholipid transfer protein (PLTP) activity in circulation reduces the phospholipid to apolipoprotein ratio in sHDL.
  • sHDL particles adapt their conformation to changes in surface area, indicating structural plasticity.