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Related Experiment Video

Updated: May 9, 2026

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Hematoxylin staining reveals a decrease in nucleolar diameter of pig oocytes before germinal vesicle breakdown.

Tadatoshi Nobata1, Hirohisa Kyougoku, Takashi Miyano

  • 1Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.

The Journal of Reproduction and Development
|July 17, 2013
PubMed
Summary
This summary is machine-generated.

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Preface.

The Journal of reproduction and development·2026
See all related articles

A new hematoxylin staining method effectively visualizes pig oocyte nucleoli. Nucleolar volume significantly decreases before germinal vesicle breakdown (GVBD) during oocyte maturation.

Area of Science:

  • Oocyte Biology
  • Cellular Morphology
  • Histology

Background:

  • Nucleoli undergo significant morphological changes during oocyte growth.
  • Conventional staining methods like aceto-orcein and immunofluorescence fail to visualize compact nucleoli in mature oocytes.

Purpose of the Study:

  • To develop a reliable hematoxylin staining technique for visualizing pig oocyte nucleoli in whole-mount preparations.
  • To examine nucleolar morphological changes during oocyte maturation using the developed staining method.

Main Methods:

  • Development of a novel whole-mount hematoxylin staining protocol for pig oocytes.
  • Microscopic observation and measurement of nucleolar diameter in growing and maturing oocytes.

Main Results:

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Last Updated: May 9, 2026

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Published on: January 7, 2016

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  • Hematoxylin successfully stained nucleoli blue in both growing and full-grown pig oocytes.
  • A statistically significant decrease in nucleolar diameter was observed in maturing oocytes prior to germinal vesicle breakdown (GVBD), from 10.7 ± 0.1 μm to 9.0 ± 0.7 μm (P<0.05).

Conclusions:

  • The developed hematoxylin staining method provides a robust tool for visualizing oocyte nucleoli.
  • Oocyte nucleolar volume diminishes preceding germinal vesicle breakdown, indicating a crucial event in maturation.