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Updated: May 9, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
Differential adaptive responses of Staphylococcus aureus to in vitro selection with different antimicrobial peptides
Tahsina Shireen1, Madhuri Singh, Tiyasa Das
1School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Abstract:
We subjected Staphylococcus aureus ATCC 29213 to serial passage in the presence of subinhibitory concentrations of magainin 2 and gramicidin D for several hundred generations. We obtained S. aureus strains with induced resistance to magainin 2 (strain 55MG) and gramicidin D (strain 55GR) that showed different phenotypic changes in membrane properties. Both exhibited a change in membrane phospholipid content and an increase in membrane rigidity, while an alteration in net charge compared to that of the control occurred only in the case of 55MG.
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