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Updated: May 9, 2026
15:32
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
Published on: August 5, 2011
The proteomics and interactomics of human erythrocytes
Steven R Goodman1, Ovidiu Daescu, David G Kakhniashvili
1Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA. goodmans@upstate.edu
Experimental Biology and Medicine (Maywood, N.J.)
|July 17, 2013
Summary
This review details advances in human erythrocyte proteome and interactome research since 2007. New proteomics and interactomics tools reveal critical protein changes in blood disorders like sickle cell disease.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- The human erythrocyte proteome and interactome are crucial for understanding red blood cell function and disease.
- Significant advancements in proteomics and interactomics have expanded our knowledge since 2007.
Purpose of the Study:
- To review recent progress in characterizing the human erythrocyte proteome and interactome.
- To highlight the application of these tools in studying blood disorders, with a focus on sickle cell disease.
Main Methods:
- Proteomics and interactomics techniques were employed to identify and analyze erythrocyte proteins.
- Comparative analyses were performed to track changes in protein profiles over time and in disease states.
Main Results:
- The number of identified unique erythrocyte proteins has increased from 751 in 2007 to 2289.
- Proteomics and interactomics have identified biomarkers for sickle cell disease severity and erythrocyte membrane alterations.
- The pharmacoproteomic effects of hydroxyurea and key protein interaction network changes in sickle cell disease were elucidated.
Conclusions:
- Proteomics and interactomics are powerful tools for advancing our understanding of erythrocyte biology and blood disorders.
- Continued research in this area holds promise for identifying novel diagnostic markers and therapeutic targets.
Related Concept Videos
01:29Structure and Function of Erythrocytes
There are between 4.2 and 6 million erythrocytes, also known as red blood cells, in every microliter of blood. These cells are small, flattened biconcave discs with centers that are depressed.
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:14Erythropoiesis
Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
01:22Lifecycle of Erythrocytes
Erythrocytes, also known as red blood cells, constantly move through blood capillaries. As a result, they damage their plasma membrane due to the continuous friction. Typically, after 100 to 120 days, erythrocytes become rigid and fragile as they wear out. As they pass through small vessels in the spleen and liver, they can get trapped and break apart into fragments.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups.
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...