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Related Concept Videos

Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
What is Cancer?02:12

What is Cancer?

Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of patients who died from...
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
Cancer02:18

Cancer

Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

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Related Experiment Video

Updated: May 9, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
12:16

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

Distinct metabolic differences between various human cancer and primary cells.

Katja Dettmer1, Franziska C Vogl, Axel P Ritter

  • 1Institute of Functional Genomics, University of Regensburg, Regensburg, Germany.

Electrophoresis
|July 17, 2013
PubMed
Summary

Cancer cells exhibit distinct metabolic profiles, differing from normal cells in nutrient utilization and metabolite secretion. This study reveals unique metabolic footprints across various cancer types, highlighting peptides

Keywords:
Mass spectrometryMetabolomicsPrimary cellsTumor cells

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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Related Experiment Videos

Last Updated: May 9, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
12:16

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Area of Science:

  • Metabolomics
  • Cancer Biology
  • Cellular Metabolism

Background:

  • Growing interest in cancer cell metabolism and secreted metabolites.
  • Tumor stroma modulation by cancer metabolites is a key area of research.

Purpose of the Study:

  • Investigate the exometabolomes of diverse cancer cell lines and primary cells.
  • Identify metabolic differences between normal and neoplastic cells.
  • Understand the role of secreted metabolites in cancer.

Main Methods:

  • Utilized liquid chromatography (LC) and gas chromatography-mass spectrometry (GC-MS) for untargeted and targeted metabolomic analysis.
  • Analyzed exometabolomes of multiple leukemia, melanoma, renal cell carcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, breast cancer, bladder carcinoma, and glioblastoma cell lines.
  • Included primary cultures of human melanocytes, hepatocytes, monocytes, CD4+, and CD8+ lymphocytes for comparison.

Main Results:

  • Unsupervised clustering grouped cell cultures primarily by tissue of origin.
  • High lactate production was a common feature across neoplastic cells.
  • Extracellular arginine and nicotinamide distinguished normal from neoplastic hepatocytes.
  • Significant differences in di- and tripeptide assimilation were observed between cell types.

Conclusions:

  • Cancer cell exometabolomes display distinct patterns related to tissue origin.
  • Peptide assimilation is crucial for meeting the high bioenergetic and biosynthetic demands of cancer cells.
  • Metabolic profiling provides insights into cancer cell behavior and potential therapeutic targets.