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Updated: May 9, 2026

Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling
Published on: January 23, 2026
[Karl Alfons Portele, Pathologist and first director of the Federal Pathologic-anatomical Museum Vienna]
Beatrix Patzak1, Eduard Winter
1Pathologisch-anatomische Sammlung im Wiener Narrenturm des Naturhistorischen Museum in Wien, Spitalgasse 2, 1090 Wien, Österreich. beatrix.patzak@nhm-wien.ac.at
Abstract:
The importance of the work of Karl Alfons Portele (1912-1993) in his position as director of the Federal Museum of Pathology is discussed. Portele was commissioned with the museum in 1946 and separated it from university in 1974. The history of the Pathologic-anatomical Museum in Vienna is closely connected with the history of pathology.
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
