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Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

Sperm Transport

Sperm Transport

The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

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Related Experiment Video

Updated: May 9, 2026

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

[Research progress in sperm mediated gene transfer technology].

Xiaoxiong Hao¹, Zheng Zhu, Mianfu Cao

¹Company Four, Third Military Medical University, Chongqing 400038, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi

|July 18, 2013

Summary

Sperm mediated gene transfer (SMGT) is a convenient and minimally invasive biotechnology for creating transgenic animals. This review details the background, development, mechanism, operation, and applications of SMGT.

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Last Updated: May 9, 2026

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

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In Vivo Microinjection and Electroporation of Mouse Testis

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Area of Science:

Biotechnology
Genetics
Animal Science

Context:

Advancements in biotechnology enable genetic modification of organisms.
Transgenic animal production is crucial for research and agriculture.
Sperm mediated gene transfer (SMGT) has emerged as a promising technique.

Purpose:

To review the background, development, mechanism, operation, and applications of sperm mediated gene transfer (SMGT).
To highlight SMGT as an effective and convenient method for producing transgenic animals.

Summary:

Sperm mediated gene transfer (SMGT) utilizes sperm cells to deliver foreign genetic material into recipient oocytes.
This method is characterized by its ease of operation and minimal cellular damage compared to other gene transfer techniques.
The review covers the foundational aspects, evolutionary progress, operational procedures, and diverse applications of SMGT technology.

Impact:

Facilitates efficient production of genetically modified animals.
Offers a less invasive alternative to traditional transgenesis methods.
Supports advancements in various fields including agriculture, medicine, and basic research.