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Updated: May 9, 2026

Detection of Abnormal Prion Protein by Immunohistochemistry
Published on: May 5, 2023
Peroxidase-linked assay for detection of antibodies against bovine leukosis virus
Clarissa C de Castro1, Cristina F Nunes, Paula F Finger
1Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil.
Abstract:
A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.
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