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Updated: May 9, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
Published on: September 10, 2018
Detection of antibodies to the 20s proteasome by ELISA
Karin Meinike Jørgensen1, Jette Lautrup Frederiksen, Christoffer Tandrup Nielsen
1Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
Abstract:
The presence of antibodies against the 20S proteasome has been correlated with diseases like multiple sclerosis (MS) and systemic lupus erythematosus (SLE) but no definite association has been established. In order to investigate this further, we optimized an ELISA for proteasome antibodies and applied it to test a total of 324 serum and plasma samples from MS patients, SLE patients, and healthy controls. Our results yield a functional and reliable assay but no correlation between the amount of proteasome antibodies present and the development of MS or SLE could be established.
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