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Updated: May 9, 2026
In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
Published on: May 12, 2023
Conformational switch for cisplatin with hemin/G-quadruplex DNAzyme supersandwich structure
Guangfeng Wang1, Xiuping He, Ling Chen
1Key Laboratory of Chem-Biosensing, Anhui Province, Anhui Normal University, Wuhu 241000, PR China; Key Laboratory of Functional Molecular Solids, Anhui Province, Anhui Normal University, Wuhu 241000, PR China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
This study introduces a novel electrochemical sensor for detecting cisplatin, a key chemotherapy drug. The sensor utilizes DNAzyme wires to achieve highly sensitive and selective cisplatin analysis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Nanotechnology
Background:
- Cisplatin is a widely used chemotherapeutic agent for various cancers.
- Accurate and sensitive detection of cisplatin is crucial for effective treatment monitoring.
- Existing detection methods may lack sensitivity or require complex procedures.
Purpose of the Study:
- To design and validate an enzyme-free electrochemical sensor for sensitive cisplatin detection.
- To leverage DNAzyme nanotechnology for signal amplification in biosensing.
- To establish a correlation between cisplatin concentration and electrochemical signal response.
Main Methods:
- Development of a hemin/G-quadruplex DNAzyme supersandwich structure.
- Enzyme-free, target-triggered amplification platform for signal enhancement.
- Electrochemical detection based on cisplatin-induced conformational changes.
- Analysis of cisplatin concentration through electrochemical signal modulation.
Main Results:
- The sensor demonstrated a wide detection range for cisplatin (0.05 to 5 μM).
- A low detection limit of 20 nM was achieved, indicating high sensitivity.
- The electrochemical signal showed a strong correlation with cisplatin concentration (R²=0.9993).
- The sensor exhibited remarkable selectivity and simplicity in design and operation.
Conclusions:
- The proposed DNAzyme-based electrochemical sensor offers a sensitive and selective method for cisplatin detection.
- The enzyme-free amplification platform provides a simple and efficient approach for biosensing applications.
- This technology holds potential for clinical applications in cancer therapy monitoring.
