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Updated: May 9, 2026
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Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Published on: May 1, 2017
An isotopically tagged azobenzene-based cleavable linker for quantitative proteomics
Yu Qian1, Julianne Martell, Nicholas J Pace
1Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467, USA.
Chembiochem : a European Journal of Chemical Biology
|July 18, 2013
Abstract:
Putting a number on it: Cleavable linkers are widely utilized in proteomics applications. In particular, the azobenzene-based linker cleaves under mild conditions that are mass-spectrometry-compatible. Here, we adapt this linker for quantitative proteomic applications by incorporating an isotopic label. These light- and heavy-tagged linkers enable the identification and quantitation of labeled peptides from multiple proteomes.
