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Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Published on: May 1, 2017
An isotopically tagged azobenzene-based cleavable linker for quantitative proteomics.
Yu Qian1, Julianne Martell, Nicholas J Pace
1Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467, USA.
Chembiochem : a European Journal of Chemical Biology
|July 18, 2013
Summary
This study introduces a new isotopic labeling method for azobenzene-based cleavable linkers, enabling precise quantification in proteomics. This technique allows for the identification and measurement of labeled peptides across different proteomes.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- Cleavable linkers are essential tools in proteomics for various applications.
- Azobenzene-based linkers offer advantages due to their cleavage under mild, mass-spectrometry-compatible conditions.
Purpose of the Study:
- To adapt azobenzene-based cleavable linkers for quantitative proteomic analysis.
- To develop a method for identifying and quantifying peptides using isotopic labeling.
Main Methods:
- Incorporation of an isotopic label into the azobenzene-based linker.
- Utilizing light- and heavy-tagged linkers for differential labeling.
- Analysis of labeled peptides using mass spectrometry.
Main Results:
- Successful adaptation of azobenzene linkers for quantitative proteomics.
- Demonstration of identification and quantitation of labeled peptides.
- Capability to analyze multiple proteomes simultaneously.
Conclusions:
- The developed isotopic labeling strategy enhances the utility of azobenzene linkers for quantitative proteomics.
- This method provides a robust approach for comparative proteomic studies.
