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Engineering multicellular logic in bacteria with metabolic wires.

Rafael Silva-Rocha1, Victor de Lorenzo

  • 1Systems Biology Program, Centro Nacional de Biotecnología CSIC , Cantoblanco-Madrid, 28049 Spain.

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Summary

Environmental bacteria pathways can be engineered for bacterial communication. Researchers created a system where one bacteria strain sends a signal to another, enabling logic operations for multicellular systems.

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Area of Science:

  • Synthetic biology
  • Microbial engineering
  • Environmental microbiology

Background:

  • Aromatic biodegradation pathways in bacteria offer enzymes, substrates, and regulators.
  • These components can be engineered to perform logic operations via cell-to-cell communication.

Purpose of the Study:

  • To demonstrate a proof-of-concept for engineered multicellular logic using bacterial communication.
  • To utilize aromatic biodegradation pathways as a source for synthetic biological circuits.

Main Methods:

  • Engineered two *Pseudomonas putida* strains for intercellular communication.
  • Utilized the TOL pathway for processing toluene and generating benzoate as a signal molecule.
  • Implemented a sender-receiver system where benzoate triggers light emission in the receiver strain.

Main Results:

  • Successfully established cell-to-cell communication between *Pseudomonas putida* strains using benzoate as a signal.
  • Demonstrated that the engineered system functions regardless of direct cell contact or liquid culture.
  • Showcased the generation of a visual output (light emission) in response to the input signal.

Conclusions:

  • Environmental metabolic pathways are valuable resources for engineering multicellular logic gates.
  • Bacterial communication systems can be designed using natural biodegradation pathways.
  • This work lays the foundation for developing more complex synthetic microbial consortia.