Aerobic biotransformation potential of a commercial mixture of naphthenic acids

The biotransformation potential of a commercial naphthenic acid (NA) mixture (NA sodium salt; TCI Chemicals) under aerobic conditions was investigated using mixed aerobic cultures developed under various levels and duration of NA exposure. A culture enriched using the commercial NA mixture as the sole carbon source degraded NAs in a range of NA concentrations, regardless of culture age and the presence of a co-substrate; however, only 28.5% of the NA-carbon was detected as CO2 while 44% was utilized for biomass growth. A fraction of the NA mixture (15-26%) was persistent under all conditions studied. In contrast, a culture fed with a degradable synthetic wastewater only (NA un-amended culture) and another culture fed with the same wastewater and 50 mg NA/L (NA-amended culture), over time lost their ability to degrade NAs. Analysis of the 16S rRNA gene based clone library revealed that 80% of the NA-enriched culture belonged to the γ-Proteobacteria class and was largely dominated by phylotypes most closely related to known NA and hydrocarbon degraders such as Pseudomonas and Microbulbifer. The results of this study indicate that although significant NA degradation is possible, only a small fraction of the NA mixture is completely mineralized to CO2. Further investigation into the biotransformation products and conditions affecting NA biodegradation under realistic refinery and environmental conditions will help to design effective treatment and bioremediation processes.