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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
Sensitive detection of melanoma metastasis using circulating microRNA expression profiles
Rie Shiiyama1, Satoshi Fukushima, Masatoshi Jinnin
1Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
Melanoma Research
|July 19, 2013
Summary
Investigating multiple microRNAs (miRNAs) reveals a more reliable diagnostic marker for metastatic melanoma. A specific combination of five miRNAs significantly improved detection sensitivity compared to individual miRNA analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Serum microRNA (miRNA) levels show potential for disease diagnosis and activity evaluation.
- Analyzing single microRNAs may lack diagnostic significance due to the vast number of identified miRNAs.
- The expression patterns and interactions of multiple miRNAs could offer a more robust biomarker for diseases like melanoma.
Purpose of the Study:
- To investigate the potential of multiple microRNA expression profiles as a diagnostic marker for melanoma, particularly metastatic melanoma.
- To explore the correlations and interactions among specific microRNAs in melanoma patients.
- To assess the diagnostic sensitivity of a combined panel of microRNAs compared to individual miRNAs.
Main Methods:
- Real-time PCR was used to evaluate the expression levels of six specific microRNAs (miR-9, miR-145, miR-150, miR-155, miR-203, and miR-205).
- Expression profiles were analyzed in two groups: 11 patients with metastatic melanoma and 16 patients without melanoma.
- Statistical analysis was performed to identify significant differences in expression and correlations between microRNAs.
Main Results:
- Significant differences in the expression of the six evaluated microRNAs were observed between patients with and without metastatic melanoma.
- Significant correlations were found between specific microRNA pairs: miR-9 and miR-205, and miR-203 and miR-205.
- A combination of five microRNAs (miR-9, miR-145, miR-150, miR-155, and miR-205) demonstrated higher sensitivity in distinguishing metastatic melanoma patients from controls than individual miRNAs.
Conclusions:
- This study is the first to report on the expression profiles of multiple microRNAs in melanoma patients.
- The findings suggest that microRNA network interactions play a role in melanoma pathogenesis.
- Utilizing multi-microRNA expression patterns holds promise for developing novel diagnostic tools for melanoma and advancing disease understanding.
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01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...