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UV-fixed-thick-blotch preparation improves sensitivity of auramine staining
M A Cejudo-García1, M J Munoz-Davila, M J Martinez-Lirola
1Microbiology Laboratory, Biotechnology Clinic Management Unit, Torrecardenas Hospital Complex, Almería, Spain.
Abstract:
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.
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