Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination of Mechanical Treatment and Enzymatic Hydrolysis During Post-Consumer Cotton Waste Processing.

ChemSusChem·2026
Same author

Retraction Note: Mechanism of HCV's resistance to IFN-α in cell culture involves expression of functional IFN-α receptor 1.

Virology journal·2026
Same author

A Method for Measuring Speech Intelligibility Using Connected Speech.

Journal of speech, language, and hearing research : JSLHR·2026
Same author

Acoustic Scene-Aware Processing and Auditory Model-Based Compensation Strategies.

Journal of the Association for Research in Otolaryngology : JARO·2026
Same author

Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia.

Cells·2026
Same author

Investigating the impact of background noise on collaborative decision-making using an individual-weighted voting model.

Cognitive research: principles and implications·2026

Related Experiment Video

Updated: May 9, 2026

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
11:26

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Published on: January 18, 2015

Eternity and functionality - rational access to physiologically relevant cell lines.

Christoph Lipps, Tobias May, Hansjörg Hauser

    Biological Chemistry
    |July 19, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Generating new cell lines has shifted from trial and error to a systematic approach. Advances in understanding molecular networks and new technologies enable the creation of physiologically relevant cell lines with controlled growth.

    More Related Videos

    Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
    08:25

    Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

    Published on: June 5, 2020

    Related Experiment Videos

    Last Updated: May 9, 2026

    Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
    11:26

    Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

    Published on: January 18, 2015

    Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
    08:25

    Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

    Published on: June 5, 2020

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biotechnology

    Background:

    • Historically, new cell line development relied on trial and error.
    • Recent advances in understanding aging, senescence, and proliferation have improved cell line generation.
    • Technological progress and deeper insights into cell differentiation and communication are key.

    Purpose of the Study:

    • To review systematic approaches for generating new cell lines with physiologic properties.
    • To highlight molecular tools for cell immortalization.
    • To discuss cell type-specific immortalization and growth control methodologies.

    Main Methods:

    • Review of molecular tools for cell immortalization.
    • Analysis of cell type-specific immortalization strategies.
    • Examination of methods for controlling newly established cell line growth.

    Main Results:

    • Cell line generation is becoming more systematic due to molecular network understanding.
    • New technological achievements support the development of physiologically relevant cell lines.
    • Focus on specific immortalization techniques and growth regulation.

    Conclusions:

    • Systematic cell line development is now feasible.
    • Molecular tools and controlled immortalization enhance cell line quality.
    • Future research directions include refining cell type-specific immortalization and growth control.