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Related Experiment Video

Updated: May 9, 2026

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

[Hybrid operation in combined pathology].

E V Drozhzhin, E R Ibragimov, D N Koval'chuk

    Angiologiia I Sosudistaia Khirurgiia = Angiology and Vascular Surgery
    |July 19, 2013
    PubMed
    Summary

    Surgical intervention effectively treated combined coronary and carotid artery atherosclerosis in a patient with coronary artery disease. This approach addressed critical blockages in both the heart and brain arteries.

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    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
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    Area of Science:

    • Cardiovascular Surgery
    • Vascular Medicine
    • Atherosclerosis Research

    Background:

    • Coronary artery disease (CAD) is a leading cause of mortality.
    • Cerebrovascular disease, often linked to atherosclerosis, poses significant stroke risk.
    • Combined coronary and carotid artery disease presents complex management challenges.

    Observation:

    • The patient presented with symptomatic coronary artery disease.
    • Concurrent atherosclerotic lesions were identified in the carotid arteries.
    • A combined surgical strategy was deemed necessary for comprehensive treatment.

    Findings:

    • Successful surgical treatment of combined atherosclerotic lesions in the coronary and carotid arteries was achieved.
    • The intervention addressed critical stenoses in both vascular beds.

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    Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
    13:05

    Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

    Published on: March 17, 2011

    Enhancing Tumor Content through Tumor Macrodissection
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  • The patient tolerated the combined procedure well.

    • Implications:

    • This case highlights the feasibility of combined surgical revascularization for patients with multivessel atherosclerotic disease.
    • Integrated treatment strategies may improve outcomes for patients with concurrent coronary and cerebrovascular disease.
    • Further research into optimizing surgical timing and techniques for combined lesions is warranted.