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Related Concept Videos

Defense Mechanism Against Infection01:26

Defense Mechanism Against Infection

Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
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Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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Related Experiment Video

Updated: May 9, 2026

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
10:35

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Published on: February 5, 2021

Vector decoys trick the immune response.

Thierry VandenDriessche1, Marinee K Chuah

  • 1Department of Gene Therapy and Regenerative Medicine, Free University of Brussels, Brussels, Belgium. thierry.vandendriessche@vub.ac.be

Science Translational Medicine
|July 19, 2013
PubMed
Summary

Preexisting antibodies can hinder adeno-associated viral vector efficacy. Using empty capsid mutant "decoys" effectively neutralizes these antibodies, enabling successful gene therapy delivery.

Area of Science:

  • Gene therapy
  • Immunology
  • Virology

Background:

  • Adeno-associated viral vectors (AAVs) are widely used in gene therapy.
  • Preexisting humoral immunity against AAVs is a significant barrier to effective gene transfer.
  • Neutralizing antibodies can prevent AAVs from reaching target cells.

Purpose of the Study:

  • To investigate a novel strategy to overcome pre-existing antibodies against adeno-associated viral vectors.
  • To determine the efficacy of empty capsid mutant decoys in neutralizing pre-existing antibodies.

Main Methods:

  • Development of empty capsid mutant adeno-associated viral vectors designed as decoys.
  • Administration of decoy vectors to subjects with pre-existing antibodies.
  • Assessment of antibody neutralization and subsequent gene transfer efficiency.

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Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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Last Updated: May 9, 2026

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Published on: January 12, 2017

Main Results:

  • Empty capsid mutant decoys successfully bound to and neutralized pre-existing antibodies against adeno-associated viral vectors.
  • Pretreatment with decoy vectors significantly enhanced the efficiency of gene transfer by subsequent therapeutic AAV vectors.
  • This strategy demonstrated a reduction in the immune barrier posed by pre-existing antibodies.

Conclusions:

  • Empty capsid mutant decoys represent a promising approach to circumvent humoral immunity against adeno-associated viral vectors.
  • This method holds potential for improving the success rates of gene therapy in a broader patient population.
  • Overcoming pre-existing antibody responses is crucial for the widespread application of adeno-associated viral vector-based therapies.