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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: May 9, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Interfacial liquid-state surface-enhanced Raman spectroscopy.

Kihoon Kim1, Hye Soo Han, Inhee Choi

  • 1Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.

Nature Communications
|July 19, 2013
PubMed
Summary
This summary is machine-generated.

Researchers achieved self-orientation of gold nanorods at a liquid interface, enabling enhanced Raman spectroscopy. This method significantly amplifies Raman signals without a solid substrate.

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Last Updated: May 9, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

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Published on: March 20, 2015

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
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Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Nanotechnology
  • Materials Science
  • Spectroscopy

Background:

  • Assembling functional nanoparticles in 3D in solution is difficult due to thermal motion.
  • Previous nanoparticle assemblies were limited to 2D solid substrates.

Purpose of the Study:

  • To develop a method for 3D nanoparticle assembly in solution.
  • To create a substrate-free surface-enhanced Raman spectroscopy (SERS) technique.

Main Methods:

  • Utilizing the immiscible oleic acid-water interface for nanoparticle self-orientation.
  • Employing dark-field imaging and Raman scattering for analysis.
  • Comparing experimental results with simulation data.

Main Results:

  • Gold nanorods spontaneously align vertically at the oleic acid-water interface.
  • This vertical alignment is a stable trapping mode, confirmed by simulations.
  • Achieved Raman signal amplification of 3-4 orders of magnitude compared to random orientations.

Conclusions:

  • The self-orientation of gold nanorods at liquid interfaces offers a novel approach for 3D assembly.
  • This method enables a highly sensitive, substrate-free SERS technique.
  • The vertical nanorod arrangement significantly enhances Raman signal detection.