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Dried human amniotic membrane does not alleviate inflammation and fibrosis in experimental strabismus surgery
Bo Young Chun1, Hong Kyun Kim, Jae Pil Shin
1Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Abstract:
Purpose. The purpose of this study was to evaluate the efficacy of dried human amniotic membrane (AM) in reducing the postoperative inflammatory response and scarring after strabismus surgery. Methods. The inflammatory response at the extraocular muscle reattachment site was analyzed after superior rectus (SR) resection in 12 rabbits. Dried human AM (Ambiodry2) was applied between the resected SR muscle plane and Tenon's capsule of the left eyes of rabbits. As a control, the right eyes of rabbits underwent SR resection only. The surgeon randomly ordered which eye gets operated first during the experiment. Two weeks later, enucleation was performed. Six sagittal sections were made for each eye at the insertion of the SR muscle. The grade of postoperative inflammation and the presence of fibrosis were evaluated in histological examinations. Results. There was no statistically significant difference in the intensity of inflammation and fibrous proliferation between the eyes treated with dried human AM after SR resection and those treated with SR resection only. Conclusions. The use of dried human AM was not effective in controlling the postoperative inflammation and scarring in rabbit eyes after extraocular muscle surgery. However, this may be due to the devitalized dry preparation of human AM (Ambiodry2), which may have lost the expected anti-inflammatory and anti-scarring properties, and further studies on humans may be necessary.
Insights
Dried human amniotic membrane (AM) did not reduce inflammation or scarring after strabismus surgery in rabbits. The study suggests the preparation method may have diminished AM
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Histology
Background:
- Strabismus surgery involves muscle reattachment, potentially leading to inflammation and scarring.
- Human amniotic membrane (AM) is explored for its potential anti-inflammatory and anti-scarring properties.
Purpose of the Study:
- To assess the efficacy of dried human AM in mitigating postoperative inflammation and scarring following strabismus surgery.
- To evaluate the impact of AM application on the extraocular muscle reattachment site.
Main Methods:
- Superior rectus resection was performed on rabbit eyes.
- Dried human AM (Ambiodry2) was applied to one eye, with the contralateral eye serving as a control.
- Histological examination assessed inflammation and fibrosis at 2 weeks post-surgery.
Main Results:
- No significant difference in inflammation or fibrosis was observed between AM-treated and control eyes.
- Dried human AM did not effectively reduce postoperative inflammation or scarring in this rabbit model.
Conclusions:
- Dried human AM (Ambiodry2) was ineffective in controlling inflammation and scarring after extraocular muscle surgery in rabbits.
- The devitalized nature of the dried AM preparation may have compromised its therapeutic potential.
- Further investigation using different AM preparations or in human subjects may be warranted.
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