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Updated: May 9, 2026
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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Published on: September 1, 2019
[Construction of Runx1 transgenic mice]
Xiaoju Tang1, Junli Li, Xiaojing Liu
1Laboratory of Cardiovascular Diseases, Research Center of Regeneration Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Summary
Researchers successfully created a Runx1 gene transgenic mouse model. This new tool enables in-depth study of the Runx1 gene
Area of Science:
- Molecular Biology
- Genetics
- Animal Models
Context:
- The Runx1 gene plays a critical role in hematopoiesis and development.
- Understanding its in vivo function is essential for various biological processes.
Purpose:
- To construct a novel transgenic mouse model specifically targeting the Runx1 gene.
- To develop a valuable tool for investigating Runx1 gene function within a living organism.
Summary:
- Mouse Runx1 cDNA was amplified and cloned into a specific vector.
- The recombinant vector was microinjected into fertilized eggs to generate transgenic mice.
- PCR and Southern blot confirmed the successful construction of the Runx1 transgenic mouse.
Impact:
- Provides a crucial in vivo tool for studying Runx1 gene function.
- Facilitates research into hematological disorders and developmental biology.
- Enables further investigation of the complex roles of Runx1 in biological systems.
